Human galactose 6-sulfatase (Gal-6S) elisa kit Instructions for use Elisa kit Specifications: 48-well configuration / 96-well configuration Standard dilution: 1.5ml × 1 bottle of enzyme standard reagent: 3 ml × 1 bottle (48) /6 ml × 1 bottle (96) [human galactose 6 sulfatase (Gal-6S) elisa kit] This reagent is only used for research use: the concentration of the standard is the abscissa and the OD is the ordinate. A standard curve is drawn on the coordinate paper, and the corresponding concentration is determined from the standard curve according to the OD value of the sample; multiplied by the dilution factor; or the linear regression equation of the standard curve is calculated by using the concentration of the standard substance and the OD value, and the OD of the sample is obtained. Substituting the value into the equation, calculating the sample concentration, and multiplying by the dilution factor, is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 part sealed bag: 1 standard: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation enzyme Standard package: 1×48 1×96 2-8°C Preservation of sample dilution: 3ml×1 bottle 6 ml×1 bottle 2-8°C Preservation of developer A: 3ml×1 bottle 6 ml×1 bottle 2 Store chromogen B solution at -8 °C: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C Preservation concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle 2-8 ° C preservation experiment principle: This kit uses the double antibody sandwich method to determine the level of human galactose 6 sulfatase (Gal-6S) in the specimen. The microporous plate was coated with purified human galactose 6-sulfatase (Gal-6S) antibody to prepare a solid phase antibody, and galactose 6-sulfatase (Gal-6S) was sequentially added to the microwell of the coated monoclonal antibody. The antibody was then combined with an HRP-labeled galactose 6-sulfatase (Gal-6S) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which was thoroughly washed and then primed with TMB. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with galactose 6 sulfatase (Gal-6S) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the concentration of human galactose 6-sulfatase (Gal-6S) in the sample was calculated from a standard curve.

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